Publikacje

  1. Bujnowicz Ł., Borek A., Kuleta P., Osyczka A., (2018) Suppression of superoxide production by a spin-spin coupling between semiquinone and Rieske cluster. FEBS Lett. in press

  2. Borek A, Ekiert R., Osyczka A, (2018) Functional flexibility of electron flow between quinol oxidation Qo site of cytochrome bc1 and cytochrome c revealed by combinatory effects of mutations in cytochrome b, iron-sulfur protein and cytochrome c1 Bioch. Biophys. Acta 1859, 754-761

  3. Pintscher S, Pietras R, Sarewicz M, Osyczka A, (2018) Electron sweep across four b-hemes of cytochrome bc1 revealed by unusual paramagnetic properties of the Qi semiquinone intermediate Bioch. Biophys. Acta 1859, 459-469

  4. Sarewicz, M. Bujnowicz, Ł., Osyczka, A. (2018) Generation of semiquinone-[2Fe-2S]+ spin-coupled center at the Qo site of cytochrome bc1 in redox-poised, illuminated photosynthetic chromatopchores from Rhodobacter capsulatus Bioch. Biophys. Acta 1859, 145-153

  5. Borek A, Ekiert R, Osyczka A, (2018) Advances in understanding mechanism and physiology of cytochromes bc. Book chapter in: Mechanisms of primary energy transduction in biology. Chemical biology no. 5. Edited by M. Wikström. Royal Society of Chemistry London pp. 192-214.

  6. Bhaduri, S., Stadnytskyi, V., Zakharov, S.D., Hasan, S.S., Bujnowicz, Ł., Sarewicz, M., Savikhin, S., Osyczka, A., Cramer, W.A., (2017) Pathways of transmembrane electron transfer in cytochrome bc complexes: dielectric heterogeneity and interheme coulombic interactions J. Phys. Chem. B 121, 975-983

  7. Sarewicz, M., Bujnowicz, Ł., Bhaduri, S., Singh, S.K., Cramer, W.A., Osyczka, A. (2017) Metastable radical state, nonreactive with oxygen, is inherent to catalysis by respiratory and photosynthetic cytochromes bc1/b6f Proc. Nat. Acad. Sci. USA 114, 1323-1328

  8. Postila, P.A., Kaszuba, K., Kuleta, P., Vattulainen, I., Sarewicz, M., Osyczka, A., Róg, T. (2016) Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex Sci. Rep. 6, 33607

  9. Kuleta, P., Sarewicz, M., Postila, P., Róg, T., Osyczka, A. (2016) Identifying involvement of Lys251/Asp252 pair in electron transfer and associated proton transfer at the quinone reduction site of Rhodobacter capsulatus cytochrome bc1 BBA Bioenergetics 1857, 10, 1661-1668

  10. Borek, A., Ekiert, R., Osyczka, A. (2016) Molekularne efekty mutacji mitochondrialnych w genie kodującym cytochrom b kompleksu III i ich wpływ na poziom produkcji wolnych rodników Postępy Biochemii 62 (2) 162-172

  11. Piertras, R., Sarewicz, M., Osyczka, A. (2016) Distinct properties of semiquinone species detected at the ubiquinol oxidation Qo site of cytochrome bc1 and their mechanistic implications J. R. Soc. Interface 13 (118)

  12. Sarewicz, M., Ekiert, R., Osyczka A. (2016) Book chapter: Inter-Monomer Electron Transfer in Cytochrome bc Complexes in: Cytochrome Complexes: Evolution, Structures, Energy Transduction, and Signaling Advances in Photosynthesis and Respiration vol. 41 (Springer)

  13. Ekiert, E., Borek, A., Kuleta, P., Czernek, J., Osyczka, A. (2016) Mitochondrial disease-related mutations at the cytochrome b-iron-sulfur protein (ISP) interface: Molecular effects on the large-scale motion of ISP and superoxide generation studied in Rhodobacter capsulatus cytochrome bc1. BBA Bioenergetics

  14. Pintscher, S., Kuleta, P., Cieluch, E., Sarewicz, M., Osyczka, A. (2016) Tuning of hemes b equilibrium redox potential is not required for cross-membrane electron transfer. J Biol Chem 291, 6872-6881 

  15. Sarewicz, M., Dutka, M., Pietras, R., Borek, A., Osyczka, A. (2015) Effect of H bond removal and changes in the position of the iron-sulphur head domain on the spin-lattice relaxation properties of the [2Fe-2S](2+) Rieske cluster in cytochrome bc(1). Phys Chem Chem Phys 17(38), 25297-25308

  16. Borek, A., Kuleta, P., Ekiert, R., Pietras, R., Sarewicz, M., Osyczka, A. (2015) Mitochondrial Disease-related Mutation G167P in Cytochrome b of Rhodobacter capsulatus Cytochrome bc1 (S151P in Human) Affects the Equilibrium Distribution of [2Fe-2S] Cluster and Generation of Superoxide. J Biol Chem 29023781-23792

  17. Sarewicz, M., Osyczka, A. (2015) Electronic Connection Between the Quinone and Cytochrome c Redox Pools and Its Role in Regulation of Mitochondrial Electron Transport and Redox Signaling. Physiol Rev. 95:219-243

  18. Pietras, R., Sarewicz, M., Osyczka, A. (2014) Molecular organization of cytochrome c2 near the binding domain of cytochrome bc1 studied by electron spin-lattice relaxation enhancement. J Phys Chem B 118: 6634–6643

  19. Ekiert, R., Czapla, M., Sarewicz, M., Osyczka, A. (2014) Hybrid fusions show that inner-monomer electron transfer robustly supports cytochrome bc1 function in vivo. Biochem Biophys Res Commun 451: 270–275

  20. Sarewicz, M., Dutka, M., Pintscher, S., Osyczka, A. (2013) Triplet State of the Semiquinone-Rieske Cluster as an Intermediate of Electronic Bifurcation Catalyzed by Cytochrome bc1. Biochemistry 52 (37), 6388–6395

  21. Pöyry, S., Cramariuc, O., Postila, P.A., Kaszuba, K., Sarewicz, M., Osyczka, A., Vattulainen, I., Róg, T.(2013)Atomistic simulations indicate cardiolipin to have an integral role in the structure of the cytochrome bc1 complex. Biochim Biophys Acta 1827(6):769-78

  22. Postila, P.A., Kaszuba, K., Sarewicz, M., Osyczka, A., Vattulainen, I., Róg, T.(2013)Key role of water in proton transfer at the Qo-site of the cytochrome bc1 complex predicted by atomistic molecular dynamics simulations. Biochim Biophys Acta 1827(6):761-8

  23. Czapla, M., Cieluch, E., Borek, A., Sarewicz, M., Osyczka, A.(2013)Catalytically-relevant electron transfer between two hemes bL in the hybrid cytochrome bc1-like complex containing a fusion of Rhodobacter sphaeroides and capsulatus cytochromes b. Biochim Biophys Acta 1827(6):751-60

  24. Kaszuba, K., Postila, P.A., Cramariuc, O., Sarewicz, M., Osyczka, A., Vattulainen, I., Róg, T. (2013) Parameterization of the prosthetic redox centers of the bacterial cytochrome bc 1 complex for atomistic molecular dynamics simulations Theor Chem Acc 132:1370

  25. Czapla, M., Sarewicz, M., and Osyczka, A. (2012) Fusing proteins as an approach to study bioenergetic enzymes and processes. Biochim Biophys Acta. 1817(10), 1847-1851

  26. Rutheford, A. W., Osyczka, A., and Rappaport, F. (2012) Back-reactions, short circuits, leaks and other energy wasteful reactions in biological electron transfer: Redox tuning to survive life in O2. FEBS Lett. 586, 603-616

  27. Czapla, M., Borek, A., Sarewicz, M., and Osyczka, A., (2012) Enzymatic activities of isolated cytochrome bc1-like complexes containing fused cytochromoe b subunits with asymmetrically inactivated segments of electron transfer chains. Biochemistry 51, 829-835

  28. Czapla, M., Borek, A., Sarewicz, M., and Osyczka, A., (2012) Fusing two cytochromes b of Rhodobacter capsulatus cytochrome bc1 using various linkers defines a set of protein templates for asymmetric mutagenesis. Protein Eng., Des. Sel. 25, 15-25

  29. Sarewicz, M., Pietras, R., Froncisz, W., and Osyczka, A. (2011) Reorientation of cytochrome c2 upon interaction with oppositely charged macromolecules probed by SR EPR: implications for the role of dipole moment to facilitate collisions in proper configuration for electron transfer. Metallomics 3, 404-409.

  30. Sarewicz, M., Borek, A., Cieluch, E., Świerczek, M., Osyczka A. (2010) Discrimination between to possible reaction sequences that create potential risk of generation of deleterious radicals by cytochrome bc1: implications for the mechanism of superoxide production. BBA Bioenergetics, 1797, 1820-1827

  31. Świerczek, M., Cieluch, E., Sarewicz, M., Borek, A., Moser, C.C., Dutton, P. L., Osyczka, A. (2010). An electronic bus bar lies in the core of cytochrome bc1. Science, 329, 451-454

  32. Cieluch, E., Pietryga K., Sarewicz, M., Osyczka, A. (2010) Visualizing changes in electron distribution in coupled chains of cytochrome bc1 by modifying barrier for electron transfer between the FeS cluster and heme c1, BBA Bioenergetics, 1797, 296-303

  33. Sarewicz, M., Dutka, M., Froncisz, W., Osyczka, A. (2009) Magnetic Interactions Sense Changes in Distance between Heme bL and the Iron−Sulfur Cluster in Cytochrome bc1, Biochemistry 48, 5708-5720

  34. Zhang, H., Chobot, S. E., Osyczka, A., Wraight, C. A., Dutton, P. L., and Moser, C. C. (2008) Quinone and non-quinone redox couples in complex III. J. Bioenerg. Biomembr. 40, 493-499.  

  35. Borek, A., Sarewicz, M., Osyczka, A. (2008) Movement of the Iron−Sulfur Head Domain of Cytochrome bc1 Transiently Opens the Catalytic Qo Site for Reaction with Oxygen, Biochemistry 47, 12365-12370

  36. Sarewicz, M., Borek, A., Daldal, F., Froncisz, W., Osyczka, A. (2008) Demonstration of Short-lived Complexes of Cytochrome c with Cytochrome bc1 by EPR Spectroscopy, J Biol Chem 283, 24826-24836

  37. Sarewicz, M., Szytuła, S., Dutka, M., Osyczka, A., Froncisz, W. (2008) Estimation of binding parameters for the protein-protein interaction using a site-directed spin labeling and EPR spectroscopy, Eur Biophys J 34, 483-493