Marcin Sarewicz, PhD

dr Marcin Sarewicz

Associate Professor

2008 Ph.D. (Biophysics); Jagiellonian University
2003 M.Sc. (Biotechnology; Biophysics); Jagiellonian University

Contact

Room: A026
Phone: 12 664 65 90
e-mail: marcin.sarewicz(at)uj.edu.pl

Scholarships

2014-2017 Stypendium Ministra dla wybitnych młodych naukowców.
2010 Nagroda zespołowa Rektora UJ za publikację w czasopiśmie Science.
2010 Nagroda Premiera za wyróżnioną pracę doktorską.

Work experience

Publications:

  1. Pintscher S, Pietras R, Sarewicz M, Osyczka A, (2018) Electron sweep across four b-hemes of cytochrome bc1 revealed by unusual paramagnetic properties of the Qi semiquinone intermediate Bioch. Biophys. Acta 1859, 459-469
  2. Sarewicz, M. Bujnowicz, Ł., Osyczka, A. (2018) Generation of semiquinone-[2Fe-2S]+ spin-coupled center at the Qo site of cytochrome bc1 in redox-poised, illuminated photosynthetic chromatopchores from Rhodobacter capsulatus Bioch. Biophys. Acta 1859, 145-153
  3. Bhaduri, S., Stadnytskyi, V., Zakharov, S.D., Hasan, S.S., Bujnowicz, Ł., Sarewicz, M., Savikhin, S., Osyczka, A., Cramer, W.A., (2017) Pathways of transmembrane electron transfer in cytochrome bc complexes: dielectric heterogeneity and interheme coulombic interactions J. Phys. Chem. B 121, 975-983<
  4. Sarewicz, M., Bujnowicz, Ł., Bhaduri, S., Singh, S.K., Cramer, W.A., Osyczka, A. (2017) Metastable radical state, nonreactive with oxygen, is inherent to catalysis by respiratory and photosynthetic cytochromes bc1/b6f Proc. Nat. Acad. Sci. USA 114, 1323-1328
  5. Postila, P.A., Kaszuba, K., Kuleta, P., Vattulainen, I., Sarewicz, M., Osyczka, A., Róg, T. (2016) Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex Sci. Rep. 6, 33607
  6. Kuleta, P., Sarewicz, M., Postila, P., Róg, T., Osyczka, A. (2016) Identifying involvement of Lys251/Asp252 pair in electron transfer and associated proton transfer at the quinone reduction site of Rhodobacter capsulatus cytochrome bc1 BBA Bioenergetics 1857, 10, 1661-1668
  7. Piertras, R., Sarewicz, M., Osyczka, A. (2016) Distinct properties of semiquinone species detected at the ubiquinol oxidation Qo site of cytochrome bc1 and their mechanistic implications J. R. Soc. Interface 13 (118)
  8. Sarewicz, M., Ekiert, R., Osyczka A. (2016) Book chapter: Inter-Monomer Electron Transfer in Cytochrome bc Complexes in: Cytochrome Complexes: Evolution, Structures, Energy Transduction, and Signaling Advances in Photosynthesis and Respiration vol. 41 (Springer)
  9. Pintscher, S., Kuleta, P., Cieluch, E., Sarewicz, M., Osyczka, A. (2016) Tuning of hemes b equilibrium redox potential is not required for cross-membrane electron transfer. J Biol Chem 291, 6872-6881 

  10. Sarewicz, M., Dutka, M., Pietras, R., Borek, A., Osyczka, A. (2015) Effect of H bond removal and changes in the position of the iron-sulphur head domain on the spin-lattice relaxation properties of the [2Fe-2S](2+) Rieske cluster in cytochrome bc(1). Phys Chem Chem Phys 17(38), 25297-25308

  11. Borek, A., Kuleta, P., Ekiert, R., Pietras, R., Sarewicz, M., Osyczka, A. (2015) Mitochondrial Disease-related Mutation G167P in Cytochrome b of Rhodobacter capsulatus Cytochrome bc1 (S151P in Human) Affects the Equilibrium Distribution of [2Fe-2S] Cluster and Generation of Superoxide. J Biol Chem 29023781-23792 
  12. Sarewicz, M., Osyczka, A. (2015) Electronic Connection Between the Quinone and Cytochrome c Redox Pools and Its Role in Regulation of Mitochondrial Electron Transport and Redox Signaling. Physiol Rev. 95:219-243

  13. Pietras, R., Sarewicz, M., Osyczka, A. (2014) Molecular organization of cytochrome c2 near the binding domain of cytochrome bc1 studied by electron spin-lattice relaxation enhancement. J Phys Chem B 118: 6634–6643

  14. Ekiert, R., Czapla, M., Sarewicz, M., Osyczka, A. (2014) Hybrid fusions show that inner-monomer electron transfer robustly supports cytochrome bc1 function in vivo. Biochem Biophys Res Commun 451: 270–275

  15. Sarewicz, M., Dutka, M., Pintscher, S., Osyczka, A. (2013) Triplet State of the Semiquinone-Rieske Cluster as an Intermediate of Electronic Bifurcation Catalyzed by Cytochrome bc1. Biochemistry 52 (37), 6388–6395

  16. Pöyry, S., Cramariuc, O., Postila, P.A., Kaszuba, K., Sarewicz, M., Osyczka, A., Vattulainen, I., Róg, T. (2013)Atomistic simulations indicate cardiolipin to have an integral role in the structure of the cytochrome bc1 complex. Biochim Biophys Acta 1827(6):769-78

  17. Postila, P.A., Kaszuba, K., Sarewicz, M., Osyczka, A., Vattulainen, I., Róg, T. (2013)Key role of water in proton transfer at the Qo-site of the cytochrome bc1 complex predicted by atomistic molecular dynamics simulations. Biochim Biophys Acta 1827(6):761-8

  18. Czapla, M., Cieluch, E., Borek, A., Sarewicz, M., Osyczka, A. (2013)Catalytically-relevant electron transfer between two hemes bL in the hybrid cytochrome bc1-like complex containing a fusion of Rhodobacter sphaeroides and capsulatus cytochromes b. Biochim Biophys Acta 1827(6):751-60

  19. Kaszuba, K., Postila, P.A., Cramariuc, O., Sarewicz, M., Osyczka, A., Vattulainen, I., Róg, T. (2013) Parameterization of the prosthetic redox centers of the bacterial cytochrome bc 1 complex for atomistic molecular dynamics simulations Theor Chem Acc 132:1370

  20. Czapla, M., Sarewicz, M., and Osyczka, A. (2012) Fusing proteins as an approach to study bioenergetic enzymes and processes. Biochim Biophys Acta. 1817(10), 1847-1851

  21. Czapla. M., Borek, A., Sarewicz, M., and Osyczka, A. (2012) Enzymatic activities of isolated cytochrome bc1-like complexes containing fused cytochrome b subunits with asymmetrically inactivated segments of electron transfer chains. Biochemistry 51, 829-835.

  22. Czapla. M., Borek, A., Sarewicz, M., and Osyczka, A. (2012) Fusing two cytochromes b of Rhodobacter capsulatus cytochrome bc1 using various linkers defines a set of protein templates for asymmetric mutagenesis. Protein Eng., Des. Sel. 25, 15-25.

  23. Sarewicz, M., Pietras, R., Froncisz, W., and Osyczka, A. (2011) Reorientation of cytochrome c2 upon interaction with oppositely charged macromolecules probed by SR EPR: implications for the role of dipole moment to facilitate collisions in proper configuration for electron transfer. Metallomics 3, 404-409.

  24. Sarewicz, M., Borek, A., Cieluch, E., Świerczek, M., and Osyczka, A. (2010) Discrimination between two possible reaction sequences that create potential risk of generation of deleterious radicals by cytochrome bc1. Implications for the mechanism of superoxide production. Biochim. Biophys. Acta 1797, 1820-1827.

  25. Świerczek, M., Cieluch, E., Sarewicz, M., Borek, A., Moser, C. C., Dutton, P. L., and Osyczka, A. (2010) An electronic bus bar lies in the core of cytochrome bc1. Science 329, 451-454.

  26. Cieluch, E., Pietryga, K., Sarewicz, M., and Osyczka, A. (2010) Visualizing changes in electron distribution in coupled chains of cytochrome bc1 by modifying barrier for electron transfer between the FeS cluster and heme c1. Biochim. Biophys. Acta 1797, 296-303.

  27. Sarewicz, M., Dutka, M., Froncisz, W., and Osyczka, A. (2009) Magnetic interactions sense changes in distance between heme bL and the iron-sulfur cluster in cytochrome bc1. Biochemistry 48, 5708-5720.

  28. Borek, A., Sarewicz, M., and Osyczka, A. (2008) Movement of the iron-sulfur head domain of cytochrome bc1 transiently opens the catalytic Qo site for reaction with oxygen. Biochemistry 47, 12365-12370.

  29. Sarewicz, M., Borek, A., Daldal, F., Froncisz, W., and Osyczka, A. (2008) Demonstration of short-lived complexes of cytochrome c with cytochrome bc1 by EPR spectroscopy. Implications for the mechanism of interprotein electron transfer. J. Biol. Chem. 283, 14826-24836.

  30. Sarewicz, M., Szytuła, S., Dutka, M., Osyczka, A., and Froncisz, W. (2008) Estimation of binding parameters for the protein-protein interaction using a site-directed spin labeling and EPR spectroscopy. Eur. Biophys. J. 37, 483-493.

  31. Dutka, M., Pyka., J., Sarewicz., M., Ptasiński, K., Szytuła, S., Froncisz, W., (2005) Electron paramagnetic resonance study of the complex dynamics of the spin label attached to cytochrome c. Current Topics in Biophysics