Arkadiusz Borek, PhD

    Research Assistant

    2015 PhD (Biophysics); Jagiellonian University
    2007 MSc (Biotechnology; Biophysics); Jagiellonian University

    Contact

    room: A024
    phone: 12 664 64 21
    e-mail: arkadiusz.borek(at)uj.edu.pl

    Scholarships

    2014-2017 Stypendium Ministra dla wybitnych młodych naukowców.
    2016 Nagroda Rektora zespołowa II stopnia za osiągnięcia naukowe.
    2011 Stypendium START
    2010 Nagroda zespołowa Rektora UJ za publikację w czasopiśmie Science.

    Work experience

    from 2005 Department of Molecular Biophysics, Jagiellonian University
    2006 intership in Medical College of Wisconsin, Milwaukee, USA

    Publications:

    1. Bujnowicz Ł., Borek A., Kuleta P., Osyczka A., (2018) Suppression of superoxide production by a spin-spin coupling between semiquinone and Rieske cluster. FEBS Lett. in press

    2. Borek A, Ekiert R., Osyczka A, (2018) Functional flexibility of electron flow between quinol oxidation Qo site of cytochrome bc1 and cytochrome c revealed by combinatory effects of mutations in cytochrome b, iron-sulfur protein and cytochrome c1 Bioch. Biophys. Acta 1859, 754-761

    3. Borek A, Ekiert R, Osyczka A, (2018) Advances in understanding mechanism and physiology of cytochromes bc. Book chapter in: Mechanisms of primary energy transduction in biology. Chemical biology no. 5. Edited by M. Wikström. Royal Society of Chemistry London pp. 192-214.

    4. Borek, A., Ekiert, R., Osyczka, A. (2016) Molekularne efekty mutacji mitochondrialnych w genie kodującym cytochrom b kompleksu III i ich wpływ na poziom produkcji wolnych rodników Postępy Biochemii 62 (2) 162-172

    5. Ekiert, E., Borek, A., Kuleta, P., Czernek, J., Osyczka, A. (2016) Mitochondrial disease-related mutations at the cytochrome b-iron-sulfur protein (ISP) interface: Molecular effects on the large-scale motion of ISP and superoxide generation studied in Rhodobacter capsulatus cytochrome bc1. BBA Bioenergetics

    6. Sarewicz, M., Dutka, M., Pietras, R., Borek, A., Osyczka, A. (2015) Effect of H bond removal and changes in the position of the iron-sulphur head domain on the spin-lattice relaxation properties of the [2Fe-2S](2+) Rieske cluster in cytochrome bc(1). Phys Chem Chem Phys 17(38), 25297-25308

    7. Borek, A., Kuleta, P., Ekiert, R., Pietras, R., Sarewicz, M., Osyczka, A. (2015) Mitochondrial Disease-related Mutation G167P in Cytochrome b of Rhodobacter capsulatus Cytochrome bc1 (S151P in Human) Affects the Equilibrium Distribution of [2Fe-2S] Cluster and Generation of Superoxide. J Biol Chem 290, 23781-23792

    8. Czapla, M., Cieluch, E., Borek, A., Sarewicz, M., Osyczka, A.(2013)Catalytically-relevant electron transfer between two hemes bL in the hybrid cytochrome bc1-like complex containing a fusion of Rhodobacter sphaeroides and capsulatus cytochromes b. Biochim Biophys Acta 1827(6):751-60

    9. Czapla, M., Borek, A., Sarewicz, M., and Osyczka, A., (2012) Enzymatic activities of isolated cytochrome bc1-like complexes containing fused cytochromoe b subunits with asymmetrically inactivated segments of electron transfer chains. Biochemistry 51, 829-835

    10. Czapla, M., Borek, A., Sarewicz, M., and Osyczka, A., (2012) Fusing two cytochromes b of Rhodobacter capsulatus cytochrome bc1 using various linkers defines a set of protein templates for asymmetric mutagenesis. Protein Eng., Des. Sel. 25, 15-25

    11. Sarewicz, M., Borek, A., Cieluch, E., Świerczek, M., Osyczka A. (2010) Discrimination between to possible reaction sequences that create potential risk of generation of deleterious radicals by cytochrome bc1: implications for the mechanism of superoxide production. BBA Bioenergetics, 1797, 1820-1827

    12. Świerczek, M., Cieluch, E., Sarewicz, M., Borek, A., Moser, C.C., Dutton, P. L., Osyczka, A. (2010). An electronic bus bar lies in the core of cytochrome bc1. Science, 329, 451-454

    13. Borek, A., Sarewicz, M., Osyczka, A. (2008) Movement of the Iron−Sulfur Head Domain of Cytochrome bc1 Transiently Opens the Catalytic Qo Site for Reaction with Oxygen, Biochemistry 47, 12365-12370

    14. Sarewicz, M., Borek, A., Daldal, F., Froncisz, W., Osyczka, A. (2008) Demonstration of Short-lived Complexes of Cytochrome c with Cytochrome bc1 by EPR Spectroscopy, J Biol Chem 283, 24826-24836